Congrès France Bioproduction

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Conference No. 1 - Facilitating Process Intensification through Mechanistic Modelling: Application to the Purification of a Plasma Protein via Aptamer Affinity Chromatography

Apr 15, 2026 — 02:15 pm - 2:35 PM
Auditorium Descartes

Conference No. 1 - Facilitating Process Intensification through Mechanistic Modelling: Application to the Purification of a Plasma Protein via Aptamer Affinity Chromatography
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Description

The manufacturing of blood-derived therapeutics remains challenging due to complex purification sequences and the need to increase the industrial productivity in order to make better use of plasma donations. Simplification of processes by the use of affinity chromatography is promising but facing reuse and scale issues to overcome. Aptamer affinity resins with its high resistance to harsh cleaning solutions will allow extensive resin use and multi-column continuous chromatography can reduce significantly size of industrial equipment. However, the experimental design of such processes is hindered by the large number of parameters that must be optimized. In this study, we developed and calibrated a mechanistic model for the purification of a plasma protein using aptamer affinity chromatography. The model integrates column hydrodynamics, binding thermodynamics, and diffusion kinetics to accurately describe system behavior. It was first applied to assess the influence of key process parameters in a single-column configuration, and subsequently extended to evaluate multi-column continuous setups. Numerical simulations were conducted for systems with three, four, and five columns, comparing their technical performance and operating costs. The optimal multi-column configuration achieved a 60% increase in productivity and a 20% reduction in operational costs relative to the best single-column setup. Overall, this modelling-driven approach provides a systematic framework for process intensification, enabling reduced experimental workload and informed decision-making in the development of robust, efficient, and cost-effective purification strategies for plasma-derived biotherapeutics.

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